Your thawing protocol could range according to your cell style, and it is usually recommended to consult with the protocol supplied by your vendor. Here is a standard protocol for freezing frozen Main cell solutions using a 37°C drinking water bath.
This allows the cells to freeze at around -1°C/minute, which can be ideal for freezing most cell types. You may also choose to use a controlled price freezer and shop the vials straight at -80°C.
Aseptic tactics: Use proper aseptic strategies to take care of sterile conditions although freezing your cells. It is a great observe to wipe down the outside of containers, for example flasks and freezing media vials, with 70% ethanol or isopropanol ahead of opening them.
The derivation of astrocytes from human pluripotent stem cells is at the moment gradual and inefficient. We exhibit that overexpression of the transcription components SOX9 and NFIB in human pluripotent stem cells swiftly and successfully yields homogeneous populations of induced astrocytes.
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